Recombinant Human STK40 Protein (His & GST Tag)

Specifications

Catalog number

PKSH030358

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

STK40 localized to both the cytoplasm and the nucleus. It is ubiquitously expressed. Mechanistically, Stk40 interacts with Rcn2, which also activates Erk1/2 to induce ExEn specification in mouse ESCs. Stk40 is able to activate the Erk/MAPK pathway and induce extraembryonic-endoderm (ExEn) differentiation in mouse ESCs. Interestingly, cells overexpressing Stk40 exclusively contribute to the ExEn layer of chimeric embryos when injected into host blastocysts. In contrast, deletion of Stk40 in ESCs markedly reduces ExEn differentiation in vitro. STK40 has a central serine/threonine protein kinase domain and is homologous to TRB-3, a protein that regulates activation of MAP kinases and inhibits NFκ B-mediated gene transcription. Similarly, overexpression of STK40 inhibits NFκ B activation triggered by TNF and also inhibits p53-mediated transcription. There are four named isoforms of STK40 that are produced as a result of alternative splicing.

Synonyms

SHIK; SgK495

UniProt

Q8N2I9

Accession Number

NP_114406

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Met 1-Lys 435

Field of Research

Signal Transduction; Cell biology; epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

76.8 kDa

Observed Molecular Weight

85 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, 3mM DTT, 0.5M Urea, 0.5mM GSH, pH 8.0

Species

Human