Recombinant Human NLK Protein (His & GST Tag)

Specifications

Catalog number

PKSH030360

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Nemo-like kinase contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and MAP kinase subfamily. It also contains a TQE activation loop motif in which autophosphorylation of the threonine residue (Thr-298) is sufficient for kinase activation. As a serine/threonine-protein kinase, nemo-like kinase regulates a number of transcription factors with key roles in cell fate determination. It is a positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs) . Nemo-like kinase also is a negative regulator of the canonical Wnt/beta-catenin signaling pathway.

Synonyms

LAK1; NLK

UniProt

Q9UBE8

Accession Number

Q9UBE8

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Val121-Glu 527

Applications

Enzyme

Field of Research

Epigenetics and nuclear signal; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 91 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 3 nmol/min/mg using MBP as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

74.1 kDa

Observed Molecular Weight

73 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol

Species

Human