Recombinant Human TLK2/PKU-ALPHA Protein

Specifications

Catalog number

PKSH030362

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Serine / threonine-protein kinase tousled-like 2; also known as PKU-alpha; Tousled-like kinase 2 and TLK2; is a nucleus protein which belongs to theprotein kinase superfamily and Ser/Thr protein kinase family. The tousled-like kinases are an evolutionarily conserved family of proteins implicated in DNA repair; DNA replication and mitosis in metazoans and plants. Their absence from the yeasts and other eukaryotic 'microbes' suggests a specific role for them in the development of multicellular organisms. Tousled-like kinase 2 / TLK2 is widely expressed. It is present in fetal placenta; liver; kidney; pancreas; heart and skeletal muscle. It is also found in adult cell lines. Tousled-like kinase 2 / TLK2 contains oneprotein kinase domain. Tousled-like kinase 2 / TLK2 is rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly.

Synonyms

HsHPK; PKU-ALPHA

UniProt

Q86UE8

Accession Number

Q86UE8-1

Expression System

Baculovirus-Insect Cells

Tag

None

Sequence

Leu 397-Asn772

Field of Research

Cell Biology, Epigenetics and Nuclear Signaling

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

43.6 kDa

Observed Molecular Weight

44 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 8.0

Species

Human