Recombinant Human TAOK3/JIK/MAP3K18 Protein (aa 1-411, His & GST Tag)

Specifications

Catalog number

PKSH030364

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Serine/threonine-protein kinase TAO3, also known as cutaneous T-cell lymphoma-associated antigen HD-CL-09, CTCL-associated antigen HD-CL-09, Dendritic cell-derived protein kinase, JNK / SAPK-inhibitory kinase, Jun kinase-inhibitory kinase, Kinase from chicken homolog A, Thousand and one amino acid protein 3, JIK, TAOK3 and MAP3K18, is cytoplasm and peripheral membrane protein which belongs to theprotein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. Protein kinases are pivotal regulators of cell signaling that modulate each other's functions and activities through site-specific phosphorylation events. TAOK3 / JIK contains oneprotein kinase domain. TAOK3 / JIK is ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver. TAOK3 / JIK inhibits the basal activity of Jun kinase. It is negatively regulated by epidermal growth factor (EGF) . When overexpressed, TAOK3 / JIK may activate ERK1 / ERK2 and JNK / SAPK.

Synonyms

DPK; JIK; MAP3K18

UniProt

Q9H2K8

Accession Number

Q9H2K8-1

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Met 1-Asp 411

Applications

Enzyme

Field of Research

Signal Transduction, Epigenetics and Nuclear Signaling

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 92 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 10 nmol/min/mg using synthetic PKCtide peptide (ERMRPRKRQGSVRRRV) as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

74.3 kDa

Observed Molecular Weight

85 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, 3mM GSH, pH 7.4, 10% glycerol

Species

Human