Recombinant Human Insulin Receptor/INSR Protein (His & GST Tag)

Specifications

Catalog number

PKSH030373

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

INSR (Insulin receptor), also known as CD220, is a transmembrane receptor that is activated by insulin. INSR belongs to theprotein kinase superfamily, and exists as a tetramer consisting of two alpha subunits and two beta subunits linked by disulfide bonds. The alpha and beta subunits are encoded by a single INSR gene, and the beta subunits pass through the cellular membrane. As the receptor for insulin with tyrosine-protein kinase activity, INSR associates with downstream mediators upon binding to insulin, including IRS1 (insulin receptor substrate 1) and phosphatidylinositol 3'-kinase (PI3K) . IRS-1 binding and phosphorylation eventually leads to an increase in the high affinity glucose transporter (Glut4) molecules on the outer membrane of insulin-responsive tissues. INSR isoform long and isoform short are expressed in the peripheral nerve, kidney, liver, striated muscle, fibroblasts and skin, and is found as a hybrid receptor with IGF1R which also binds IGF1 in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibrobasts, spleen and placenta. Defects in Insulin Receptor/INSR are the cause of Rabson-Mendenhall syndrome (Mendenhall syndrome), insulin resistance (Ins resistance), leprechaunism (Donohue syndrome), and familial hyperinsulinemic hypoglycemia 5 (HHF5) . It may also be associated with noninsulin-dependent diabetes mellitus (NIDDM) .

Synonyms

CD220; HHF5; Insulin Receptor

UniProt

P06213

Accession Number

NP_000199.2

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Gly 989-Ser 1382

Applications

Enzyme

Field of Research

Signal Transduction; Cardiovascular; Neuroscience; Cancer; Developmental Biology; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 92 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 45 nmol/min/mg using Poly (Ala, Glu, Lys, Tyr) 6:2:5:1 as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

72.3 kDa

Observed Molecular Weight

70 kDa

Formulation

Supplied as sterile 50mM Tris, 100mM NaCI, pH 7.5, 25% glycerol, 1mM TCEP,0.5mM GSH

Species

Human