Recombinant Human Hexokinase-3/HK3 Protein (His & GST Tag)

Specifications

Catalog number

PKSH030376

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Hexokinase-3, also known as Hexokinase type III, HKIII and HK3, is a protein which belongs to thehexokinase family. Hexokinase-3 / HK3 is anenzymewhich in humans is encoded by theHK2gene. Hexokinases phosphorylate glucoseto produceglucose 6-phosphate, committing glucose to theglycolytic pathway. In mammalian tissues hexokinase exists as four isoenzymes encoded by distinct genes. These proteins are homologous and are organized in two homologous domains, with the exception of hexokinase type IV which has only one. This organization is believed to be the result of a duplication and tandem fusion event involving the gene encoding for the ancestral hexokinase. The gene encodes hexokinase-3. Similar to hexokinases-1 and hexokinases-2, thisallostericenzyme is inhibited by its product glucose 6-phosphate.

Synonyms

HKIII; HXK3

UniProt

P52790

Accession Number

P52790

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Met 1-Val 923

Field of Research

Signal Transduction; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

127 kDa

Observed Molecular Weight

115 kDa

Formulation

Supplied as sterile solution of 50mM Tris, 100mM NaCl, pH 7.4

Citation 01

https://www.sciencedirect.com/science/article/pii/S0300908422000177

Species

Human