Recombinant Human RSK3/RPS6KA2 Protein (GST Tag)

Specifications

Catalog number

PKSH030377

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ribosomal protein S6 kinase alpha-2, also known as 90 kDa ribosomal protein S6 kinase 2, MAP kinase-activated protein kinase 1c, MAPK-activated protein kinase 1c, Ribosomal S6 kinase 3, RSK-3, RPS6KA2 and MAPKAPK1C, is a nucleus protein which belongs to theprotein kinase superfamily, AGC Ser/Thr protein kinase family and S6 kinase subfamily. RPS6KA2 / RSK-3 is expressed in many tissues. Highest expression is in lung and skeletal muscle. The expression of RPS6KA2 reduced proliferation, caused G1 arrest, increased apoptosis, reduced levels of phosphorylated extracellular signal-regulated kinase and altered other cell cycle proteins. RPS6KA2 / RSK-3 contains oneAGC-kinase C-terminal domain and twoprotein kinase domains. It forms a complex with either ERK1 or ERK2 in quiescent cells. It transiently dissociates following mitogenic stimulation. RPS6KA2 / RSK-3 is a serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. RPS6KA1, RPS6KA2, RPS6KB1, RPS6KB2, and PDK1 are involved in several pathways central to the carcinogenic process, including regulation of cell growth, insulin, and inflammation.

Synonyms

HU-2; MAPKAPK1C; RSK; RSK3; S6K-alpha; S6K-alpha2; p90-RSK3; pp90RSK3

UniProt

Q15349

Accession Number

Q15349-1

Expression System

Baculovirus-Insect Cells

Tag

N-GST

Sequence

Met 1-Leu 733

Applications

Enzyme

Field of Research

Epigenetics and Nuclear Signaling, Signal Transduction

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 84 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 41 nmol/min/mg using synthetic RSK peptide (KRRRLSSLRA) as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

110 kDa

Observed Molecular Weight

110 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.0, 20mM GSH

Species

Human