Recombinant Human FGFR2/CD332 Protein (aa 400-821, His & GST Tag)

Specifications

Catalog number

PKSH030379

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

FGFR2, also known as CD332, belongs to the fibroblast growth factor receptor subfamily where amino acid sequence is highly conserved between members and throughout evolution. FGFR2 acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. It is required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. FGFR2 plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. It also promotes cell proliferation in keratinocytes and imature osteoblasts, but promotes apoptosis in differentiated osteoblasts. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal CD332 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1. Defects in CD3322 are the cause of Crouzon syndrome, Jackson-Weiss syndrome, Apert syndrome, Pfeiffer syndrome, Beare-Stevenson cutis gyrata syndrome, familial scaphocephaly syndrome, lacrimo-auriculo-dento-digital syndrome and Antley-Bixler syndrome without genital anomalies or disordered steroidogenesis.

Synonyms

BBDS; BEK; BFR-1; CD332; CEK3; CFD1; ECT1; JWS; K-SAM; KGFR; TK14; TK25

UniProt

P21802

Accession Number

NP_000132.3

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Met 400-Thr 821

Applications

ELISA; Enzyme

Field of Research

Signal Transduction; Cardiovascular; Neuroscience; Cancer; Developmental Biology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 92 % as determined by reducing SDS-PAGE.

Bioactivity

1. The specific activity was determined to be 28 nmol/min/mg using Poly (Glu:Tyr) 4:1 as substrate. 2. Immobilized recombinant human FGFR2 (aa 400-821) at 10 μg/ml (100 μl/well) can bind biotinylated human FGF acidic with a linear range of 15.6-250 ng/ml. 3. Immobilized recombinant human FGFR2 (aa 400-821) at 10 μg/ml (100 μl/well) can bind biotinylated human FGF basic with a linear range of 0.16-1. 25 μg/ml.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

75.7 kDa

Observed Molecular Weight

68 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol

Species

Human