Recombinant Human TPL2/MAP3K8/MEKK8 Protein (GST Tag)

Specifications

Catalog number

PKSH030380

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mitogen-activated protein kinase kinase kinase 8, also known as Cancer Osaka thyroid oncogene, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, Tumor progression locus 2 and MAP3K8, is a cytoplasm protein which belongs to theprotein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K8 is expressed in several normal tissues and human tumor-derived cell lines. Isoform1of MAP3K8 is activated specifically during the S and G2/M phases of the cell cycle. MAP3K8 is required for TLR4 activation of the MEK/ERK pathway. It is able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. MAP3K8 plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein. MAP3K8 oncogene linked to human endometrial carcinoma suggesting that it may be another molecule involved in human endometrial cancer. MAP3K8 may also be an important mediator of intracellular mechanotransduction in human bone marrow-derived mesenchymal stem cells (MSCs) .

Synonyms

AURA2; COT; EST; ESTF; MEKK8; TPL2; Tpl-2; c-COT

UniProt

P41279

Accession Number

P41279-1

Expression System

Baculovirus-Insect Cells

Tag

N-GST

Sequence

Met 30-Arg 397

Field of Research

Signal Transduction, Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 91 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

68.0 kDa

Observed Molecular Weight

68 kDa

Formulation

Supplied as sterile solution of 50mM Tris, 100mM NaCl, 0.5mM PMSF, 0.5mM GSH, pH 8.0

Species

Human