Recombinant Human BLK Protein (GST Tag)

Specifications

Catalog number

PKSH030381

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tyrosine-protein kinase Blk, also known as B lymphocyte kinase, p55-Blk and BLK, is a member of theprotein kinase superfamily, Tyr protein kinase family and SRC subfamily. BLK / p55-Blk is expressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles. BLK / p55-Blk is a src-family protein tyrosine kinase specifically expressed in B-lineage cells of mice. The early onset of Blk expression during B-cell development in the bone marrow and the high expression levels of Blk in mature B cells suggest a possible important role of Blk in B-cell physiology. It is a modulator of beta-cells function, acting through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose. Defects in BLK are a cause of maturity-onset diabetes of the young type 11 which is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.

Synonyms

B Lymphocyte Kinase; BLK; MODY11; Tyrosine-Protein Kinase Blk; p55-Blk

UniProt

P51451

Accession Number

NP_001706.2

Expression System

Baculovirus-Insect Cells

Tag

N-GST

Sequence

Met 1-Pro 505

Applications

Enzyme

Field of Research

Cell biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 88 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 17.4 nmol/min/mg using Poly (Glu, Tyr) 4:1 peptide as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

84 kDa

Observed Molecular Weight

84 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, 5mM GSH, pH 7.4

Species

Human