Recombinant Human DAPK3/ZIPK Protein (GST Tag)

Specifications

Catalog number

PKSH030384

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Death-associated protein kinase 3, also known as DAP kinase 3, ZIP-kinase, DAPK3 and ZIPK, is a nucleus and cytoplasm protein which belongs to theprotein kinase superfamily, CAMK Ser/Thr protein kinase family and DAP kinase subfamily. DAPK3 / ZIPK contains oneprotein kinase domain. It is a serine/threonine kinase which acts as a positive regulator of apoptosis. It phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. DAPK3 / ZIPK is a homodimer or forms heterodimers with ATF4. Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. It also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. DAPK3 / ZIPK regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. It is involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.

Synonyms

DLK; ZIP; ZIPK

UniProt

O43293

Accession Number

NP_001339.1

Expression System

Baculovirus-Insect Cells

Tag

N-GST

Sequence

Met 1-Arg 454

Applications

Enzyme

Field of Research

Signal Transduction; Cell biology; Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 5 nmol/min/mg using MBP as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

79.0 kDa

Observed Molecular Weight

70 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10mM GSH, pH 7.4

Species

Human