Recombinant Human SRC Kinase/c-SRC Protein (His & GST Tag)

Specifications

Catalog number

PKSH030385

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is a constitutively active as opposed to normal SRC (C-SRC) . Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.

Synonyms

ASV; SRC1; c-SRC; p60-Src

UniProt

P12931

Accession Number

P12931-1

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Met 1-Leu 536

Applications

Enzyme

Field of Research

Signal Transduction, Epigenetics and Nuclear Signaling, Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be > 80 nmol/min/mg using Poly (Glu:Tyr) 4:1 as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

87.7 kDa

Observed Molecular Weight

81 kDa

Formulation

Supplied as sterile solution of 50mM Tris, 100mM NaCl, pH 8.0, 20% glycerol, 0.3mM DTT

Species

Human