Recombinant Human IRAK4/IRAK-4 Protein (His Tag)

Specifications

Catalog number

PKSH030387-01

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-1 receptor-associated kinase 4, also known as Renal carcinoma antigen NY-REN-64, IRAK-4 and IRAK4, is a member of the protein kinase superfamily, TKL Ser/Thr protein kinase family and Pelle subfamily. IRAK4 contains onedeath domain and oneprotein kinase domain. IRAK4 is required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. It also phosphorylates IRAK1. A member of the IL-1 receptor (IL-1R) -associated kinase (IRAK) family, IRAK4, has been shown to play an essential role in Toll-like receptor (TLR) -mediated signaling. IL-1-mediated IRAK4 kinase activity in T cells is essential for induction of IL-23R expression, Th17 differentiation, and autoimmune disease. Pharmacological blocking of IRAK4 kinase activity will retain some levels of host defence, while reducing the levels and duration of inflammatory responses, which should provide beneficial therapies for sepsis and chronic inflammatory diseases. Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) which is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD. Defects in IRAK4 are also the cause of IRAK4 deficiency which causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children.

Synonyms

IPD1; IRAK-4; NY-REN-64; REN64

UniProt

Q9NWZ3

Accession Number

Q9NWZ3-1

Expression System

Baculovirus-Insect Cells

Tag

N-His

Sequence

Met 1-Ser 460

Applications

Enzyme

Field of Research

Immunology, Signal Transduction, Cardiovascular

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 92 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 169 nmol/min/mg using MBP as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

53.8 kDa

Observed Molecular Weight

48 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, 0.5mM PMSF, pH 7.4

Species

Human