Recombinant Human DDR1 Kinase/MCK10 Protein (aa 444-913, His & GST Tag)

Specifications

Catalog number

PKSH030389

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 10 (MCK10), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK10/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK10/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.

Synonyms

CAK; CD167; DDR; EDDR1; HGK2; MCK10; NEP; NTRK4; PTK3; PTK3A; RTK6; TRKE

UniProt

Q08345

Accession Number

Q08345-1

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Arg444-Val913

Applications

Enzyme

Field of Research

Neuroscience, Cancer, Cardiovascular

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 89 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 2. 75 nmol/min/mg using synthetic AXLtide peptide (CKKSRGDYMTMQIG) as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

80.0 kDa

Observed Molecular Weight

80 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 3mM DTT

Species

Human