Recombinant Human CAMK1D Protein (GST Tag)

Specifications

Catalog number

PKSH030390

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Calcium/calmodulin-dependent protein kinase orCaM kinases are serine/threonine-specific protein kinasesthat are primarily regulated by the Calcium/calmodulin complex. These kinases show a memory effect on activation. CaM kinases activity can outlast the intracellular calcium transient that is needed to activate it. Inneurons, this property is important for the induction of synaptic plasticity. Pharmacological inhibition of CaM kinases II blocks the induction oflong-term potentiation. Upon activation, CaM kinases II phosphorylates postsynaptic glutamate receptors and changes the electrical properties of the synapse. Calcium/calmodulin-dependent protein kinase type 1D, also known as CaM kinase I delta, CaM kinase ID, CaMKI-like protein kinase, CKLiK and CAMK1D, is a member of theprotein kinase superfamily and CaMK subfamily. It contains oneprotein kinase domain. CAMK1D is broadly expressed. It is highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes. Engineered overexpression of CAMK1D in non-tumorigenic breast epithelial cells led to increased cell proliferation, and molecular and phenotypic alterations indicative of epithelial-mesenchymal transition (EMT), including loss of cell-cell adhesions and increased cell migration and invasion. CAMK1D is a potential therapeutic target with particular relevance to clinically unfavorable basal-like tumors.

Synonyms

CAMK1D; CKLiK; CaM kinase I delta; CaM-K1; CaMK1 delta; CaMKI-like protein kinase; Calcium/calmodulin-dependent protein kinase type 1D

UniProt

Q5SQQ7

Accession Number

NP_705718.1

Expression System

Baculovirus-Insect Cells

Tag

N-GST

Sequence

Met 1-Lys 385

Applications

Enzyme

Field of Research

Signal Transduction; Cell biology; Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 80 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 70 nmol/min/mg using Autocamtide-2 synthetic peptide (KKALRRQETVDAL-amide) as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

69.0 kDa

Observed Molecular Weight

60 kDa

Formulation

Supplied as sterile solution of 50mM Tris, 100mM NaCl, 0.5mM Reduced Glutathione, pH 8.0

Species

Human