Recombinant Human TRIB2/TRB2 Protein (His & GST Tag)

Specifications

Catalog number

PKSH030394

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tribbles homolog 2, also known as TRB-2, and Trib2, is a member of the protein kinase superfamily and Tribbles subfamily (Trib1, Trib2, Trib3) . The identification of tribbles as regulators of signal processing systems and physiological processes, including development, together with their potential involvement in diabetes and cancer, has generated considerable interest in these proteins. Tribbles have been reported to regulate activation of a number of intracellular signalling pathways with roles extending from mitosis and cell activation to apoptosis and modulation of gene expression. Tribbles controls the timing of mitosis in the prospective mesoderm, allowing cell-shape changes to be completed. This mechanism for coordinating cell division and cell-shape changes may have helped Drosophila to evolve its mode of rapid early development. Trib2 was identified as a downregulated transcript in leukemic cells undergoing growth arrest. Trib2-transduced bone marrow cells exhibited a growth advantage and readily established factor-dependent cell lines. Trib2-reconstituted mice uniformly developed fatal transplantable acute myelogenous leukemia (AML) .

Synonyms

C5FW; FLJ57420; GS3955; TRB2; TRIB2

UniProt

Q92519

Accession Number

NP_067675.1

Expression System

Baculovirus-Insect Cells

Tag

N-His & C-GST

Sequence

Met 1-Asn 343

Field of Research

Signal Transduction; Cardiovascular; Cancer; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

66.0 kDa

Formulation

Supplied as sterile solution of 50mM Tris, 100mM NaCl, 0.5mM PMSF, 0.5mM GSH, pH 8.0

Species

Human