Recombinant Human c-MET/HGFR Protein (aa 956-1390, His & GST Tag)

Specifications

Catalog number

PKSH030396

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.

Synonyms

AUTS9; DFNB97; HGFR; RCCP2; c-Met

UniProt

P08581

Accession Number

P08581-1

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Lys956-Ser1390

Applications

ELISA; Enzyme

Field of Research

Signal Transduction, Epigenetics and Nuclear Signaling, Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

1. The specific activity was determined to be 10 nmol/min/mg using MBP as substrate. 2. Immobilized human HGFR (aa 956-1390) at 10 μg/ml (100 μl/well) can bind biotinylated human HGF-his with a linear range of 15.6-125 ng/ml.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

76.8 kDa

Observed Molecular Weight

68 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 3mM DTT

Species

Human