Recombinant Human PTK6/Brk Protein (GST Tag)

Specifications

Catalog number

PKSH030398

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tyrosine kinase (PTKs) is a protein that carry out tyrosine phosphorylation, which play a fundamental role in cell proliferation, survival, adhesion, and motility and have also been demenstrated to mediate malignant cell transformation. Overexpression of this protein in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Two classes of PTKs are present in cells: the transmembrane receptor PTKs and the non-receptor PTKs. Tyrosine kinase (PTKs) -6/ BRK is a cytoplasmic non-receptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Tyrosine kinase (PTKs) -6/ BRK has been shown to undergo autophosphorylation. It has been found that the constitutive expression of the tyrosine kinase (PTKs) -6/ BRK is in a large proportion of cutaneous T-cell lymphomas and other transformed T- and B-cell populations. State BRK expression was also induced in normal T-cells. In clinical, the cytoplasmic tyrosine kinase PTK6 (BRK) shows elevated expression in approximately two-thirds of primary breast tumours, and is implicated in EGF receptor-dependent signalling and epithelial tumorigenesis.

Synonyms

BRK

UniProt

Q13882

Accession Number

NP_005966.1

Expression System

Baculovirus-Insect Cells

Tag

N-GST

Sequence

Met 1-Thr 451

Field of Research

Signal Transduction; Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 89 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

78.0 kDa

Observed Molecular Weight

70 kDa

Formulation

Supplied as sterile solution of 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol

Species

Human