Recombinant Human CSNK1A1/CKI-alpha/CK1 Protein (GST Tag)

Specifications

Catalog number

PKSH030400

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Casein kinase I isoform alpha, also known as CKI-alpha, CK1 and CSNK1A1, is a cytoplasm protein which belongs to theprotein kinase superfamily, CK1 Ser/Thr protein kinase family and casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. High expression of CSNK2A1, or concomitantly high expression of CSNK2A1, are independent prognostic factors of poor survival in NSCLC patients. CSNK2A1 are useful prognosis markers in non-small cell lung cancer (NSCLC) patients after complete resection, independent of lymph node metastasis status. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. It phosphorylates CTNNB1 at 'Ser-45'. CSNK1A1 may play a role in segregating chromosomes during mitosis.

Synonyms

CK1; CK1a; CKIa; HEL-S-77p; HLCDGP1; PRO2975

UniProt

P48729

Accession Number

P48729-1

Expression System

Baculovirus-Insect Cells

Tag

N-GST

Sequence

Met 1-Phe 337

Applications

Enzyme

Field of Research

Signal Transduction, Stem Cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 30 nmol/min/mg using casein as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

65.2 kDa

Observed Molecular Weight

62 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, 2mM GSH, pH 7.4

Species

Human