Recombinant Human CSNK2A1/CK2A1 Protein (GST Tag)

Specifications

Catalog number

PKSH030405

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Casein kinase II subunit alpha, also known as CK II alpha, CSNK2A1 and CK2A1, is a member of the protein kinase superfamily, Ser / Thr protein kinase family and CK2 subfamily. Casein kinase II (CSNK2A1) is a serine / threonine protein kinase that phosphorylates acidic proteins such as casein. This kinase is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. Casein kinase II (CSNK2A1) is a constitutively active, ubiquitously expressed serine / threonine protein kinase that is thought to have a regulatory function in cell proliferation, cell differentiation and apoptosis. CSNK2A1 functions as a tetrameric complex consisting of two regulatory beta-subunits and two catalytic units (alpha and alpha') in a homomeric or heteromeric conformation. Whilst the alpha- and alpha'-subunits are catalytically identical, proteins that regulate CSNK2A1, such as cdc2 and Hsp90, preferentially bind to the alpha and not the alpha'-subunit. CSNK2A1 can phosphorylate a number of key intracellular signaling proteins implicated in tumor suppression (p53 and PTEN) and tumorigenesis (myc, jun, NF-kappaB) . CSNK2A1 is also thought to influence Wnt signaling via beta-catenin phosphorylation and the PI 3-K signaling pathway via th phosphorylation of Akt.

Synonyms

CK2A1; CKII; CSNK2A3

UniProt

P68400

Accession Number

NP_808227.1

Expression System

Baculovirus-Insect Cells

Tag

N-GST

Sequence

Met 1-Gln 391

Applications

Enzyme

Field of Research

Signal Transduction; Cell biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 93 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 9 nmol/min/mg using casein as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

71.4 kDa

Observed Molecular Weight

65 kDa

Formulation

Supplied as sterile solution of 50mM Tris, 100mM NaCl, 0.5mM PMSF, 0.5mM GSH, pH 8.0

Species

Human