Recombinant Human FGFR1/CD331 Protein (His & GST Tag)

Specifications

Catalog number

PKSH030406

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

FGFR1; also known as CD331; belongs to the fibroblast growth factor receptor subfamily where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. Fibroblast growth factors (FGFs) (FGF1 - 10 and 16 - 23) are mitogenic signaling molecules that have roles in angiogenesis; wound healing; cell migration; neural outgrowth and embryonic development. FGFs bind heparan sulfate glycosaminoglycans; which facilitates dimerization (activation) of FGF receptors. FGFR1 is a full-length representative protein consists of an extracellular region; composed of three immunoglobulin-like domains; a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of FGFR1 interacts with fibroblast growth factors; setting in motion a cascade of downstream signals; ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. CD331 can be detected in astrocytoma; neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines; however isoform 17; isoform 18 and isoform 19 are not detected in these cells. Defects in FGFR1 are a cause of Pfeiffer syndrome ; idiopathic hypogonadotropic hypogonadism; Kallmann syndrome type 2; osteoglophonic dysplasia and trigonocephaly non-syndromic.

Synonyms

BFGFR; CD331; CEK; FGFBR; FGFR-1; FLG; FLT-2; FLT2; HBGFR; HH2; HRTFDS; KAL2; N-SAM; OGD; bFGF-R-1

UniProt

P11362

Accession Number

NP_075594.1

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Gly 311-Arg 731

Field of Research

Signal Transduction; Cardiovascular; Neuroscience; Cancer; epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 93 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

75.0 kDa

Observed Molecular Weight

75 kDa

Formulation

Supplied as sterile solution of 50mM Tris, 100mM NaCl, pH 8.0, 20% glycerol, 0.3mM DTT

Species

Human