Recombinant Human Bruton Tyrosine Kinase/BTK Kinase Protein (His Tag)

Specifications

Catalog number

PKSH030407

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Bruton's tyrosine kinase (or BTK) is a type of kinase protein expressed in B lymphocytes and T cells. BTK contains a PH domain which binds phosphatidylinositol (3,4,5) -trisphosphate (PIP3) . After binding to PIP3, BTK is induced to phosphorylate phospholipase C, which in turn hydrolyzes PIP2 into two second messagers, IP3 and DAG, which then modulate the activity of downstream proteins during B-cell signaling. Btk is also found implicated in the primary immunodeficiency disease X-linked agammaglobulinemia (Bruton's agammaglobulinemia) . BTK played a key role in B-cell maturation as well as mast cell activation through the high-affinity IgE receptor. Patients with X-linked agammaglobulinemia have normal pre-B cell populations in their bone marrow but these B-cells can not mature and enter the circulation.

Synonyms

AGMX1; AT; ATK; BPK; IMD1; PSCTK1; XLA

UniProt

Q06187

Accession Number

NP_000052.1

Expression System

Baculovirus-Insect Cells

Tag

C-His

Sequence

Met 1-Ser 659

Applications

Enzyme

Field of Research

Signal Transduction; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 115 nmol/min/mg using Poly (Glu, Tyr) 4:1 peptide as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

77.8 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.0

Species

Human