Recombinant Human PKC iota/PRKCI Protein (GST Tag)

Specifications

Catalog number

PKSH030411

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein kinase C iota type, also known as Atypical protein kinase C-lambda/iota, aPKC-lambda/iota and PRKCI, is a cytoplasm, membrane and nucleus protein which belongs to theprotein kinase superfamily, AGC Ser/Thr protein kinase family and PKC subfamily. PRKCI contains oneAGC-kinase C-terminal domain, oneOPR domain, onephorbol-ester/DAG-type zinc finger and oneprotein kinase domain. PRKCI is predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. It is highly expressed in non-small cell lung cancers. PRKCI is a calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase. It may play a role in the secretory response to nutrients. PRKCI is involved in cell polarization processes and the formation of epithelial tight junctions. It is implicated in the activation of several signaling pathways including Ras, c-Src and NF-kappa-B pathways. PRKCI functions in both pro- and anti-apoptotic pathways. It functions in the RAC1/ERK signaling required for transformed growth. PRKCI plays a role in microtubule dynamics through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs) . PRKCI might be a target for novel lipid activators that are elevated during nutrient-stimulated insulin secretion.

Synonyms

DXS1179E; PKCI; nPKC-iota

UniProt

P41743

Accession Number

NP_002731.4

Expression System

Baculovirus-Insect Cells

Tag

C-GST

Sequence

Met 10-Val 596

Field of Research

Signal Transduction

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 88 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

93.5 kDa

Observed Molecular Weight

100 kDa

Formulation

Supplied as sterile solution of 50mM Tris, 100mM NaCl, 0.5mM GSH, 0.5mM PMSF, 10% glycerol, pH 7.4

Species

Human