Recombinant Human MKK6 Protein (His & GST Tag)

Specifications

Catalog number

PKSH030415

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Dual specificity mitogen-activated protein kinase kinase 6, also known as MAP kinase kinase 6, MAPKK 6, MAPK / ERK kinase 6, SAPKK3, MAP2K6, and MKK6, is a protein that belongs to the protein kinase superfamily, STE Ser / Thr protein kinase family and MAP kinase kinase subfamily. MAP2K6 / MKK6 contains one protein kinase domain. Mitogen-activated protein kinases are members of a conserved cascade of kinases involved in many signal transduction pathways. They stimulate phosphorylation of transcription factors in response to extracellular signals such as growth factors, cytokines, ultraviolet light, and stress-inducing agents. MAP2K6 / MKK6 exists in a variety of alternatively spliced isoforms with distinct patterns of tissue expression. Isoform 2 of MAP2K6 / MKK6 is only expressed in skeletal muscle. Isoform 1 of MAP2K6 / MKK6 is expressed in skeletal muscle, heart, and to a lesser extent in liver or pancreas.

Synonyms

MAP2K6 ; MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3; SAPKK3

UniProt

P52564

Accession Number

P52564-1

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Met 1-Asp 334

Field of Research

Cell Biology, Signal Transduction, Immunology, Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 80 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

65.3 kDa

Observed Molecular Weight

60 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 8.0

Species

Human