Recombinant Human PTP1B/PTPN1 Protein (His Tag)

Specifications

Catalog number

PKSH030416

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

PTP1B, also known as PTPN1, belongs to the protein-tyrosine phosphatase (PTP) family. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTP1B contains 1 tyrosine-protein phosphatase domain and is expressed in many tissues. PTP1B is localized to the cytoplasmic face of the endoplasmic reticulum. PTP1B was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of PTP1B in cell growth control, and cell response to IFN stimulation.

Synonyms

PTP1B; PTPN1

UniProt

P18031

Accession Number

NP_002818.1

Expression System

E.coli

Tag

N-His

Sequence

Glu 2-Asn 321

Applications

Enzyme

Field of Research

Signal Transduction; Cardiovascular

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor (aa988-998) phosphopeptide substrate, R&D Systems, Catalog # ES006. The specific activity is > 15 nmoles/min/μg.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

38 kDa

Observed Molecular Weight

38 kDa

Formulation

Supplied as sterile solution of PBS, pH 7.4

Species

Human