Recombinant Human MERTK/MER Protein (His&GST Tag)

Specifications

Catalog number

PKSH030417

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Proto-oncogene tyrosine-protein kinase MER (MERTK) is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains; two Ig-like C2-type (immunoglobulin-like) domains; and one tyrosine kinase domain. MERTK is localized in membrane and is no expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. This protein is highly expressed in testis; ovary; prostate; lung; and kidney; with lower expression in spleen; small intestine; colon; and liver. MERTK regulates many physiological processes including cell survival; migration; differentiation; and phagocytosis of apoptotic cells (efferocytosis) . Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells; platelet aggregation; cytoskeleton reorganization and engulfment. MERTK plays also an important role in inhibition of Toll-like receptors (TLRs) -mediated innate immune response by activating STAT1; which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. Defects in MERTK are the cause of retinitis pigmentosa type 38.

Synonyms

MER; MERTK; Mer; Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK; Tyrosine-protein kinase Mer

UniProt

Q12866

Accession Number

Q12866

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Glu 578-Tyr 872

Field of Research

Signal Transduction; Cell biology; Neuroscience; immunology; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 92 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

62.0 kDa

Observed Molecular Weight

50 kDa

Formulation

Supplied as sterile solution of 50mM Tris, 100mM NaCl, pH 7.4, 20% glycerol, 0.3mM DTT

Species

Human