Recombinant Human EphB4/HTK Protein (aa 563-987, His & GST Tag)

Specifications

Catalog number

PKSH030418

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.

Synonyms

HTK; MYK1; TYRO11

UniProt

P54760

Accession Number

P54760

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Leu563-Tyr987

Applications

Enzyme

Field of Research

Signal Transduction; Cardiovascular; Neuroscience; Cancer; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 47 nmol/min/mg using Poly (Glu:Tyr) 4:1 as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

75.2 kDa

Observed Molecular Weight

66 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 8.0, 3mM DTT, 10% glycerol

Species

Human