Recombinant Human ALK-2/ACVR1 Protein (Baculovirus, His Tag)

Specifications

Catalog number

PKSH030419-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

ALK-2; also termed as ACVR1; was initially identified as an activin type I receptor because of its ability to bind activin in concert with ActRII or ActRIIB. ALK-2 is also identified as a BMP type I receptor. It has been demonstrated that ALK-2 forms complex with either the BMP-2/7-bound BMPR-II or ACVR2A /ACVR2B. ALK-1 and ALK-2 presenting in the yeast Saccharomyces cerevisiae are two haspin homologues. Both ALK-1 and ALK-2 exhibit a weak auto-kinase activity in vitro; and are phosphoproteins in vivo. ALK-1 and ALK-2 levels peak in mitosis and late-S/G2. Control of protein stability plays a major role in ALK-2 regulation. The half-life of ALK-2 is particularly short in G1. Overexpression of ALK-2; but not of ALK-1; causes a mitotic arrest; which is correlated to the kinase activity of the protein. This suggests a role for ALK-2 in the control of mitosis. Endoglin is phosphorylated on cytosolic domain threonine residues by the TGF-beta type I receptors ALK-2 and ALK-5 in prostate cancer cells. Endoglin did not inhibit cell migration in the presence of constitutively active ALK-2. Defects in ALK-2 are a cause of fibrodysplasia ossificans progressiva (FOP) .

Synonyms

ACTR-I; ACVR1; ACVR1A; ACVRLK2; ALK-2; ALK2; Activin Receptor Type I; Activin Receptor Type-1; Activin Receptor-Like Kinase 2; FOP; SKR1; Serine/Threonine-Protein Kinase Receptor R1; TGF-B Superfamily Receptor Type I; TSR-I

UniProt

Q04771

Accession Number

Q04771

Expression System

Baculovirus-Insect Cells

Tag

C-His

Sequence

Met 1-Val 124

Field of Research

Signal Transduction; epigenetics and nuclear signal; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 93 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

12.8 kDa

Observed Molecular Weight

17 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol

Species

Human