Recombinant Human CSF1R/CD115 Protein (His & GST Tag)

Specifications

Catalog number

PKSH030422

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

M-CSFR encoded by the proto-oncogene c-fms is the receptor for colony stimulating factor 1 (CSF1R), a cytokine involved in the proliferation, differentiation, and activation of macrophages. This cell surface glycoprotein is consisted by an extracellular ligand-binding domain, a single membrane-spanning segment, and an intracellular tyrosine kinase domain. Binding of CSF1 activates the receptor kinase, leading to "autophosphorylation" of receptor subunits and the concomitant phosphorylation of a series of cellular proteins on tyrosine residues. CSF1R is a tyrosine kinase receptor that is absolutely required for macrophage differentiation and thus occupies a central role in hematopoiesis. CSF1 and its receptor (CSF1R, product of c-fms proto-oncogene) were initially implicated as essential for normal monocyte development as well as for trophoblastic implantation. This apparent role for CSF1/CSF1R in normal mammary gland development is very intriguing because this receptor/ligand pair has also been found to be important in the biology of breast cancer in which abnormal expression of CSF1 and its receptor correlates with tumor cell invasiveness and adverse clinical prognosis. Tumor cell expression of CSF1R is under the control of several steroid hormones (glucocorticoids and progestins) and the binding of several bHLH transcription factors, while tumor cell expression of CSF-1 appears to be regulated by other hormones, some of which are involved in normal lactogenic differentiation.

Synonyms

C-FMS; CD115; CSF-1 receptor; CSF-1-R; CSF-1R; CSF1R; FIM2; FMS; HDLS; M-CSF-R; MCSF Receptor ; Macrophage colony-stimulating factor 1 receptor; Proto-oncogene c-Fms

UniProt

P07333

Accession Number

NP_005202.2

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Lys 543-Cys 972

Field of Research

Signal Transduction; Cardiovascular; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 82 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

76 kDa

Observed Molecular Weight

75 kDa

Formulation

Supplied as sterile solution of 50mM Tris, 150mM NaCl, pH 7.4, 20% glycerol, 0.3mM DTT

Species

Human