Recombinant Human EGFR/ErbB1 Protein (aa 668-1210, His&GST Tag)

Specifications

Catalog number

PKSH030428

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

As a member of the epidermal growth factor receptor (EGFR) family; EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF; amphiregulin; TGF-α ; betacellulin; etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation; survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization; the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK; PI3K/PKB and STAT) . In addition; EGFR signaling also has been shown to exert action on carcinogenesis and disease progression; and thus EGFR protein is proposed as a target for cancer therapy currently.

Synonyms

ERBB; ERBB1; HER1; NISBD2; PIG61; mENA

UniProt

P00533

Accession Number

NP_005219

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Met 668-Ala 1210

Applications

Enzyme

Field of Research

Signal Transduction; Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 105 nmol/min/mg using Poly (Glu:Tyr) 4:1 as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

89.1 kDa

Formulation

Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4

Species

Human