Recombinant Human IL-23 (IL23A&IL12B Heterodimer) Protein (His Tag)

Specifications

Catalog number

PKSH030445-01

Size / count

5 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells.

Abbreviation

IL-23 (IL23A&IL12B

Synonyms

CLMF p40; IL-12 subunit p40; IL-23; IL-23A; IL-23p19; IL23P19; NKSF2; P19; SGRF

Accession Number

Q9NPF7&NP_002178.2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Pro189&Met 1-Ser 28

Applications

Cell culture; ELISA

Field of Research

Microbiology; Cancer; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

1. Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA. 2. Immobilized human IL23A-His+IL12B-His at 10 μg/ml (100 μl/well) can bind human IL23R-Fc. The EC50 of human IL23R-Fc is 0.28-0.66 μg/ml. 3. Immobilized human IL23A-His+IL12B-His at 10 μg/ml (100 μl/well) can bind Cynomolgus IL23R-Fc. The EC50 of Cynomolgus IL23R-Fc is 0.14-0.35 μg/ml. 4. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 4-20 ng/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

20.1&36.2 kDa

Observed Molecular Weight

22&45 kDa

Species

Human