Recombinant Human EpCAM/TROP-1 Protein (His Tag)

Specifications

Catalog number

PKSH030447-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â … transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.

Synonyms

DIAR5; EGP-2; EGP314; EGP40; ESA; HNPCC8; KS1/4; KSA; M4S1; MIC18; MK-1; TACSTD1; TROP-1; TROP1

Accession Number

NP_002345.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Lys265

Applications

Cell culture

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 96 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When cells are added to EpCAM-His coated plates (1. 25μg/mL, 100μL/well), approximately > 40% will adhere specifically after 30 minutes at 37°C.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

29 kDa

Observed Molecular Weight

36 kDa

Species

Human