Recombinant Human Thioredoxin/TXN Protein

Specifications

Catalog number

PKSH030450-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Thioredoxin; also known as ATL-derived factor; Surface-associated sulphydryl protein; SASP and TXN; is a nucleus; cytoplasm and secreted protein which belongs to thethioredoxin family. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals. Thioredoxin / TXN participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin / TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins; and thereby contributes to the response to intracellular nitric oxide. Thioredoxin / TXN nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO) ; and thereby inhibits caspase-3 activity. Thioredoxin / TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

Synonyms

ADF; ATL-Derived Factor; SASP; Surface-Associated Sulphydryl Protein; TRDX; TRX; TRX1; TXN; Thioredoxin; Trx

UniProt

P10599

Accession Number

P10599

Expression System

E.coli

Tag

None

Sequence

Met 1-Val 105

Applications

Enzyme

Field of Research

Signal Transduction; metabolism

Endotoxin

Please contact us for more information.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to catalyze the reduction of insulin. The reaction leads toprecipitation, which can be measured by absorbance at 650 nm. The specific activity is 5-10 A650/min/mg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

11.7 kDa

Observed Molecular Weight

14 kDa

Species

Human