Recombinant Human DPP4/DPPIV/CD26 Protein (Fc Tag)

Specifications

Catalog number

PKSH030456

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Dipeptidyl peptidase-4 (DPP4) or adenosine deaminase complexing protein 2 (ADCP 2) or T-cell activation antigen CD26 is a serine exopeptidase belonging to the S9B protein family that cleaves X-proline dipeptides from the N-terminus of polypeptides, such as chemokines, neuropeptides, and peptide hormones. The enzyme is a type II transmembrane glycoprotein, expressed on the surface of many cell types. It is also present in serum and other body fluids in a truncated form (sCD26/DPPIV) . The soluble CD26 (sCD26) as a tumour marker for the detection of colorectal cancer (CRC) and advanced adenomas. As both a regulatory enzyme and a signalling factor, DPP4 has been evaluated and described in many studies. DPP4 inhibition results in increased blood concentration of the incretin hormones glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP) . This causes an increase in glucose-dependent stimulation, resulting in a lowering of blood glucose levels. Recent studies have shown that DPP4 inhibitors can induce a significant reduction in glycosylated haemoglobin (HbA (1c) ) levels, either as monotherapy or as a combination with other antidiabetic agents. Research has also demonstrated that DPP4 inhibitors portray a very low risk of hypoglycaemia development, and are a new pharmacological class of drugs for treating Type 2 diabetes.

Synonyms

ADABP; ADCP-2; Adenosine deaminase complexing protein 2; DPP IV; Dipeptidyl peptidase 4; Dipeptidyl peptidase IV; T-cell activation antigen CD26

UniProt

P27487

Accession Number

NP_001926.2

Expression System

HEK293 Cells

Tag

N-hFc

Sequence

Asn 29-Pro 766

Applications

ELISA

Field of Research

Cell biology; Cancer; immunology; metabolism; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

1. Measured by its ability to bind recombinant Cynomolgus CXCL12 in a functional ELISA. 2. Measured by its ability to bind recombinant Human SDF1b in a functional ELISA. 3. Using the Octet RED System, the affinity constant (Kd) of human Fc-DPPIV bound to Spike (HCoV-EMC/2012) was 11 nM. 4. Using the Octet RED System, the affinity constant (Kd) of human Fc-DPPIV bound to Spike (HCoV-EMC/2012) was 32 nM. 5. Using the Octet RED System, the affinity constant (Kd) of human Fc-DPPIV bound to Spike (HCoV-EMC/2012) (ECD, aa 1-1297) was 43 nM. 6. Using the Octet RED System, the affinity constant (Kd) of human Fc-DPPIV bound to Spike-His (aa 1-760) was 12 nM.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

112 kDa

Observed Molecular Weight

120-130 kDa

Species

Human