Recombinant Human CD3D & CD3E Heterodimer Protein

Specifications

Catalog number

PKSH030479-01

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

T-cell surface glycoprotein CD3D & CD3E, also known as CD3 delta & CD3 epsilon chain, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Synonyms

CD3D; CD3d; T-Cell Receptor T3 Delta Chain; T-Cell Surface Glycoprotein CD3 Delta Chain; T3D

UniProt

P04234; P07766

Accession Number

P04234-1&P07766-1

Expression System

HEK293 Cells

Tag

C-Flag-hFc

Sequence

Met 1-Ala105&Met 1-Asp126

Applications

ELISA

Field of Research

Immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized human CD3D & CD3E Heterodimer Protein at 10 μg/ml (100 μl/well) can bind OKT3-mIgG2a. The EC50 of OKT3-mIgG2a is 0.07-0.17 μg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

37.5&40 kDa

Observed Molecular Weight

45-50 kDa

Species

Human