Recombinant Human NPC1 Protein (His & FLAG Tag)

Specifications

Catalog number

PKSH030488

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Niemann-Pick C1 (NPC1), a host receptor involved in the envelope glycoprotein (GP) -mediated entry of filoviruses into cells, is believed to be a major determinant of cell susceptibility to filovirus infection. Niemann-Pick C1 (NPC1), a membrane protein of lysosomes, is required for the export of cholesterol derived from receptor-mediated endocytosis of LDL.The NPC1 protein is a multipass transmembrane protein whose deficiency causes the autosomal recessive lipid storage disorder Niemann-Pick type C1. NPC1 localizes predominantly to late endosomes and has a dileucine motif located within a small cytoplasmic tail thought to target the protein to this location.Niemann-Pick disease Type C1 (NPC1) is a rare progressive neurodegenerative disorder caused by mutations in the NPC1 gene. On the cellular level NPC1 mutations lead to an accumulation of cholesterol and gangliosides.

Synonyms

NPC

UniProt

O15118

Accession Number

NP_000262.2

Expression System

HEK293 Cells

Tag

N-His-Flag

Sequence

Arg372-Phe622

Field of Research

Signal Transduction; Cardiovascular; Neuroscience; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

32.0 kDa

Species

Human