Recombinant Human EphA1 Protein (Fc Tag)

Specifications

Catalog number

PKSH030520

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

EPHA1 or EPH receptor A1 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. An important role of Eph receptors and their ligands ephrins is to mediate cell-contact-dependent repulsion. Eph receptors and ephrins also act at boundaries to channel neuronal growth cones along specific pathways, restrict the migration of neural crest cells, and via bidirectional signaling prevent intermingling between hindbrain segments. Eph receptors and ephrins can also trigger an adhesive response of endothelial cells and are required for the remodeling of blood vessels. Eph receptors and ephrins have emerged as key regulators of the repulsion and adhesion of cells that underlie the establishment, maintainence, and remodeling of patterns of cellular organization. The ephrins and Eph receptors are implicated as positional labels that may guide the development of neural topographic maps.

Synonyms

EPH; EPH tyrosine kinase; EPH tyrosine kinase 1; EPHA1; EPHT; EPHT1; Ephrin type-A receptor 1; Erythropoietin-producing hepatoma receptor; Tyrosine-protein kinase receptor EPH; hEpha1

UniProt

P21709

Accession Number

EAL23789.1

Expression System

HEK293 Cells

Tag

C-hFc

Sequence

Met 1-Glu547

Applications

ELISA

Field of Research

Cardiovascular, Neuroscience

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human EFNA1-His at 10 μg/ml (100 μl /well) can bind human EPHA1-Fc, The EC50 of human EPHA1-Fc is 10-30 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

83.3 kDa

Species

Human