Recombinant Human Biotinidase/BTD Protein (His Tag)

Specifications

Catalog number

PKSH030529

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Biotinidase, also known as biotinase and BTD, is a ubiquitous mammalian cell enzyme which expressed at high levels in the liver, serum, and kidney. Its primary function is to cleave biotin from biocytin, preserving the pool of biotin for use as a cofactor for biotin dependent enzymes, namely the 4 human carboxylases. Biotinidase also recycles biotin from enzymes in the body that use it as a helper component in order to function. These enzymes, known ascarboxylases, are important in the processing offats, carbohydrates, andproteins. Biotin is attached to these carboxylase enzymes through anamino acid (the building material of proteins) calledlysine, forming a complex calledbiocytin.

Synonyms

BTD

UniProt

P43251

Accession Number

P43251

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Asp543

Applications

Enzyme

Field of Research

Signal Transduction; Neuroscience; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to hydrolyze biocytin to lysine and biotin. The specific activity is > 500pmol/min/μg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

58.2 kDa

Observed Molecular Weight

66-76 kDa

Species

Human