Recombinant Human PPP3R1 Protein (His Tag)

Specifications

Catalog number

PKSH030576

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

PPP3R1 belongs to the calcineurin regulatory subunit family. It is a regulatory subunit of calcineurin. Calcineurin is composed of two subunits: calcineurin A (CnA) and calcineurin B (CnB) . Dephosphorylation of the nuclear factor of activated T-cells (NF-AT) by Calcineurin is essential for NF-AT activation; nuclear translocation; and early gene expression in T-cells. PPP3R1 is a Ser/Thr-specific calcium and calmodulin-dependent protein phosphatase which takes a vital part in the T cell activation pathway. PPP3R1 is involved in protein dephosphorylation; NFAT protein import into nucleus (ortholog) and epithelial to mesenchymal transition (ortholog) . It participates in calcineurin signaling pathway; mitogen activated protein kinase signaling pathway. PPP3R1 interacts with (+) -pilocarpine; 2;4-dinitrotoluene and ammonium chloride. It contains four EF-hand domains and four functional calcium-binding sites. PPP3R1 play an improtant role in the T cell activation pathway.

Synonyms

CNA2; CNB; Calcineurin Subunit B Type 1; PPP3R1; Protein Phosphatase 2B Regulatory Subunit 1; Protein Phosphatase 3 Regulatory Subunit B Alpha Osoform 1

UniProt

P63098

Accession Number

P63098

Expression System

Baculovirus-Insect Cells

Tag

N-His

Sequence

Gly2-Val170

Applications

BLI

Field of Research

Signal Transduction; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 87 % as determined by reducing SDS-PAGE.

Bioactivity

Using the Octet RED System, the affinity constant (Kd) of human PPP3R1-His bound to Human PPIA-His was 6 nM.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

21.4 kDa

Observed Molecular Weight

20 kDa

Species

Human