Recombinant Human PPP3CA/CALNA Protein (His Tag)

Specifications

Catalog number

PKSH030577

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

PPP3CA, also known as protein phosphatase 2B, is a member of the PPP phosphatase family, PP-2B subfamily. It is the alpha catalytic subunit of protein phosphatase 2B (PP2B) . PP2B is a holoenzyme that is comprised of a catalytic subunit associated with regulatory subunits. It is a calcium regulated enzyme that is activated by calmodulin and participates in the signaling cascades involved in development of the nervous, cardiovascular, and musculoskeletal systems. PPP3CA activates the T cells of the immune system and can be blocked by drugs. It also activates NFATc (a transcription factor) by dephosphorylating it. The activated NFATc is subsequently translocated into the nucleus, where it upregulates the expression of interleukin 2. PPP3CA interacts with CRTC2, MYOZ1, MYOZ2 and MYOZ3. It also interacts with DNM1L. The interaction dephosphorylates DNM1L and regulates its translocation to mitochondria.

Synonyms

CALN; CALNA; CALNA1; CCN1; CNA1; PPP2B

UniProt

Q08209

Accession Number

Q08209-1

Expression System

Baculovirus-Insect Cells

Tag

N-His

Sequence

Ser2-Gln521

Applications

BLI

Field of Research

Immunology, Signal Transduction

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 94 % as determined by reducing SDS-PAGE.

Bioactivity

Using the Octet RED System, the affinity constant (Kd) of human PPPP3CA-His bound to Human PPIA-His was 0.9 nM.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

60.8 kDa

Observed Molecular Weight

60 kDa

Species

Human