Recombinant Human CLPS/Colipase Protein (His Tag)

Specifications

Catalog number

PKSH030591

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Colipase belongs to the colipase family. Structural studies of the complex and of colipase alone have revealed the functionality of its architecture. It is a small protein with five conserved disulphide bonds. Structural analogies have been recognised between a developmental protein, the pancreatic lipase C-terminal domain, the N-terminal domains of lipoxygenases and the C-terminal domain of alpha-toxin. Colipase can only be detected in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Colipase allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase. Colipase is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilising as active conformation and considerably increasing the overall hydrophobic binding site.

Synonyms

CLPS

UniProt

P04118

Accession Number

P04118

Expression System

Baculovirus-Insect Cells

Tag

C-His

Sequence

Met 1-Gln 112

Applications

ELISA

Field of Research

Signal Transduction; Cardiovascular; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human CLPS-His at 10μg/mL (100μL/well) can bind biotinylated human PNLIP-His. The EC50 of biotinylated human PNLIP-His is 0.57-1. 33μg/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

11.5 kDa

Observed Molecular Weight

12 kDa

Species

Human