Recombinant Human CALR/Calreticulin Protein (Fc Tag)

Specifications

Catalog number

PKSH030607

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Calreticulin is a multifunctional protein. It acts as a main Ca (2+) -binding (storage) protein in the lumen of the endoplasmic reticulum. Calreticulin binds Ca2+ ions (a second messenger in signal transduction) ; rendering it inactive. The Ca2+ is bound with low affinity; but high capacity; and can be released on a signal. Located in storage compartments associated with the endoplasmic reticulum; calreticulin also binds to misfolded proteins and prevents them from being exported from the endoplasmic reticulum to the golgi apparatus. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin reduces the binding of androgen receptor to its hormone-responsive DNA element and inhibits androgen receptor and retinoic acid receptor transcriptional activities in vivo; as well as retinoic acid-induced neuronal differentiation. Therefore; calreticulin acts as a significant modulator of the regulation of gene transcription by nuclear hormone receptors.

Synonyms

CRT; HEL-S-99n; RO; SSA; cC1qR

UniProt

P27797

Accession Number

P27797

Expression System

HEK293 Cells

Tag

C-hFc

Sequence

Met 1-Ala413

Field of Research

Signal Transduction; Tags & Cell Markers

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

73.0 kDa

Observed Molecular Weight

96&38 kDa

Species

Human