Recombinant Human TPP1/CLN2 Protein (His Tag)

Specifications

Catalog number

PKSH030613

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tripeptidyl-peptidase 1 (TPP1 / CLN2) is a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. TPP1 / CLN2 May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Defects in TPP1 / CLN2 are the cause of neuronal ceroid lipofuscinosis type 2 (CLN2), a form of neuronal ceroid lipofuscinosis which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy.

Synonyms

CLN2; Cell Growth-Inhibiting Gene 1 Protein; GIG1; LPIC; Lysosomal Pepstatin-Insensitive Protease; SCAR7; TPP-1; TPP1; Tripeptidyl Aminopeptidase; Tripeptidyl-Peptidase 1

UniProt

O14773

Accession Number

AAH14863.1

Expression System

Baculovirus-Insect Cells

Tag

C-His

Sequence

Met 1-Pro 563

Applications

Enzyme

Field of Research

Neuroscience, Cell Biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by the cleavage of AlaAlaPheAMC. The specific activity is > 850 pmoles/min/μg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

60.7 kDa

Observed Molecular Weight

60 kDa

Species

Human