Recombinant Human TMED1 Protein (His Tag)

Specifications

Catalog number

PKSH030660

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

TMED1 belongs to the EMP24/GP25L family. It contains 1 GOLD domain and is widely expressed. TMED1 binds to its receptor IL1RL1 and results in the activation of DNA binding by nuclear factor NF-kappa-B or transcription from the IL8 promoter and most likely requires other proteins to elicit these activities. Dendritic cells from Peyer's patches (but not from spleen) express TMED1 in response to treatment with LPS. TMED1 may play a role in vesicular protein trafficking; mainly in the early secretory pathway. It may act as a cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and may be involved in vesicle coat formation at the cytoplasmic side.

Synonyms

IL1RL1LG; Il1rl1l; Tp24

UniProt

Q13445

Accession Number

Q13445

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Asn194

Applications

ELISA

Field of Research

Cell biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its binding ability in a functional ELISA. 2. Immobilized human TMED1-His at 10μg/mL (100μL/well) can bind human IL1R4-Fc, the EC50 of human IL1R4-Fc is 8-50ng/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

20.6 kDa

Observed Molecular Weight

28 kDa

Species

Human