Recombinant Human CDCP1/CD318 Protein (aa 1-343, His Tag)

Specifications

Catalog number

PKSH030670

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CDCP1 contains three extracellular CUB domains. It is a putative stem cell marker that is highly expressed in some human cancer cells and in both; typical and atypical (cancerous) colons. It interacts with CDH2/N-cadherin; CDH3/P-cadherin; SDC1/syndecan-1; SDC4/syndecan-4 and the serine protease ST14/MT-SP1. It also interacts with SRC and PRKCG/protein kinase C gamma. CDCP1 is taken as a key regulator of EGF/EGFR-induced cell migration. It has been shown that signaling via EGF/EGFR induces migration of ovarian cancer Caov3 and OVCA420 cells with concomitant up-regulation of CDCP1 mRNA and protein. Consistent with a role in cell migration CDCP1 relocates from cell-cell junctions to punctate structures on filopodia after activation of EGFR. It may be involved in cell adhesion and cell matrix association. It also may play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. It has been taken as a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets.

Synonyms

CD318; CUB domain-containing protein 1; Membrane glycoprotein gp140; SIMA135; Subtractive immunization M plus HEp3-associated 135 kDa protein; TRASK; Transmembrane and associated with src kinases

UniProt

Q9H5V8

Accession Number

Q9H5V8-3

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Glu343

Field of Research

Tags & Cell Markers, Stem Cells, Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

36.4 kDa

Species

Human