Recombinant Human SerpinB3/SCCA1 Protein (His Tag)

Specifications

Catalog number

PKSH030698

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

SERPINB3, also known as SCCA-1, belongs to the serpin family. Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. The acronym serpin was originally coined because many serpins inhibit chymotrypsin-like serine proteases. SERPINB3 is expressed in some hepatocellular carcinoma (at protein level) . Its expression is closely related to cellular differentiation in both normal and malignant squamous cells. It seems to also be secreted in plasma by cancerous cells but at a low level. SERPINB3 significantly attenuates apoptosis by contrasting cytochrome c release from the mitochondria and by antichemotactic effect for NK cells. It may act as a protease inhibitor to modulate the host immune response against tumor cells and may be involved in the malignant behavior of squamous cell carcinoma cells.

Synonyms

HsT1196; Protein T4-A; SCC; SCCA-1; SCCA1; Serpin B3; Squamous cell carcinoma antigen 1; T4-A; clade B (ovalbumin) ; member 3; serine (or cysteine) proteinase inhibitor; serpin peptidase inhibitor

UniProt

P29508

Accession Number

AAB20405.1

Expression System

Baculovirus-Insect Cells

Tag

N-His

Sequence

Asn 2-Pro 390

Applications

Enzyme

Field of Research

Cell Biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenice peptide substrate Z-LR-AMC, R&D Systems, Catalog # ES008.The IC50 is < 5 nM.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

46.7 kDa

Observed Molecular Weight

43 kDa

Species

Human