Recombinant Human UBE2D4 Protein (His Tag)

Specifications

Catalog number

PKSH030702

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

UBE2D4 is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation; ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction; and thereby the fate of the substrate; is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space; a highly sophisticated conjugation machinery has been developed. In a sequential manner; ubiquitin becomes activated by an ubiquitin-activating enzyme (E1) ; which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s) . Next; ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system; wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans. It has been identified the UBE2D family (UBE2D1-4) as E2 partners for IDOL that support both autoubiquitination and IDOL-dependent ubiquitination of the LDLR in a cell-free system.

Synonyms

HBUCE1; UBCH5D; UBE2D4; Ubiquitin Carrier Protein D4; Ubiquitin-Conjugating Enzyme E2 D4; Ubiquitin-Protein Ligase D4

UniProt

Q9Y2X8

Accession Number

Q9Y2X8

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Met 147

Field of Research

Cell biology; epigenetics and nuclear signal

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

17.5 kDa

Observed Molecular Weight

18.5 kDa

Species

Human