Recombinant Human FcERI/FCER1A Protein (His Tag)

Specifications

Catalog number

PKSH030706-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

FcERI, also known as FCER1A, is the alpha subunit of the immunoglobulin epsilon receptor (IgE receptor) . IgE receptor is a high affinity IgE receptor which plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma. The allergic response occurs when 2 or more IgE receptors are crosslinked via IgE molecules that in turn are bound to an allergen (antigen) molecule. A perturbation occurs that brings about the release of histamine and proteases from the granules in the cytoplasm of the mast cell and leads to the synthesis of prostaglandins and leukotrienes--potent effectors of the hypersensitivity response. IgE receptor is comprised of an alpha subunit (FcERI), a beta subunit, and two gamma subunits. FcERI is glycosylated and contains 2 Ig-like (immunoglobulin-like) domains.

Synonyms

FCE1A; FcERI

UniProt

P12319

Accession Number

P12319

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Gln205

Applications

ELISA

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized Recombinant Human FcERI / FCER1A Protein (ECD, His Tag) at 2 μg/mL (100 μl/well) can bind Recombinant Human IgE-Fc Protein (Constant Domain 3&4, His Tag), the EC50 is 10-30 ng/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

22.5 kDa

Observed Molecular Weight

42-48 kDa

Species

Human