Recombinant Human SUMO1 Protein (His Tag)

Specifications

Catalog number

PKSH030730

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Small ubiquitin-like modifier protein (SUMO) modification is a highly dynamic process, catalyzed by SUMO-specific activating (E1), conjugating (E2) and ligating (E3) enzymes, and reversed by a family of SUMO-specific proteases (SENPs) . Small ubiquitin-like modifier 1 (SUMO1) is a member of the superfamily of ubiquitin-like proteins. Despite its structural similarity with ubiquitin, SUMO1 does not seem to play any role in protein degradation. SUMO1 plays an important role in modulation of NOX activity required for ROS generation. SUMO1 haploinsufficiency results in cleft lip and palate in animal models. SUMO1 gene variation in human non-syndromic cleft lip with or without cleft palate (NSCLP) development. SUMO-1 may be useful as a novel target for therapy in oral squamous cell carcinoma (SCC) as well as a clinical indicator for tumor recurrence together with Mdm2.

Synonyms

DAP1; GAP-Modifying Protein 1; GMP1; OFC10; SENP2; SMT3; SMT3 Homolog 3; SMT3C; SMT3H3; SUMO-1; SUMO1; Sentrin; Small Ubiquitin-Related Modifier 1; Smt3C; UBL1; Ubiquitin-Homology Domain Protein PIC1; Ubiquitin-Like Protein; Ubiquitin-Like Protein SMT3C

Accession Number

AAH66306.1

Expression System

E.coli

Tag

N-His

Sequence

Ser 2-Val 101

Applications

Enzyme

Endotoxin

Please contact us for more information.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to be proteolytically processed by SENP1. > 50% of 1 μg Recombinant Human (rh) SUMO1 is cleaved by < 10 ng of recombinant human SENP.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

12.4 kDa

Species

Human