Recombinant Human PGA4/Pepsinogen A Protein (His Tag)

Specifications

Catalog number

PKSH030741

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

PGA4 (Pepsinogen 4; group I) ; or Pepsinogen A; is a member of the peptidase A1 family. Pepsin is expressed as a pro-form zymogen; pepsinogen; whose primary structure has an additional 44 amino acids. Pepsin is stored as pepsinogen so it will only be released when needed; and does not digest the body's own proteins in the stomach's lining. Five types of zymogens of pepsins; gastric digestive proteinases; are known: pepsinogens A; B; and F; progastricsin; and prochymosin. There are two major groups of pepsinogen; namely pepsinogen A (PGA) and pepsinogen C (PGC) (or progastricsin) ; and each frequently has isozymogens. The PGA3; PGA4 and PGA5 genes encode identical human pepsinogen A enzymes.

Synonyms

FLJ58952; FLJ77962; PGA3; PGA4; PGA5

UniProt

P0DJD7

Accession Number

NP-001073276.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Ala 388

Applications

Enzyme

Field of Research

Signal Transduction, Cell Biology, Metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp) -NH2, AnaSpec, Catalog # 27114.The specific activity is > 10, 000 pmoles/min/μg. (Activation description: The enzyme achieves its activity under acidic pH)

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

41.7 kDa

Observed Molecular Weight

45 kDa

Species

Human